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axio imager.z2 slide scanning fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axio imager.z2 slide scanning fluorescence microscope
    Axio Imager.Z2 Slide Scanning Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+imager%2Ez2+slide+scanning+fluorescence+microscope/axio+microscope+observer+z1+%CE%B2/pm40057214-85-7-13
    Average 90 stars, based on 1 article reviews
    axio imager.z2 slide scanning fluorescence microscope - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer’s disease
    Article Snippet: Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer's disease
    Article Snippet: Multiplex uorescence immunohistochemistry image acquisition and computational reconstruction Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning uorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized lter sets (Semrock) optimized to detect the following uorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal Page 8/53 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: Neurovascular injury with complement activation and inflammation in COVID-19.
    Article Snippet: Imageswere acquired fromwhole specimen sections using theAxio Imager.Z2 slide scanning fluorescence microscope (Carl Zeiss AG) equipped with a ×20/0.8 Plan-Apochromat (Phase-2) objective (Carl Zeiss AG), an ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies) and eight customized filter sets (Semrock) to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 594, Alexa Fluor 647, IRDye 680LT and IRDye 800CW.

    Article Title: TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression.
    Article Snippet: The nuclear pellet was collected by aspirating ~100 μl from the interface and transferring to a 2.5ml Eppendorf tube.

    Article Title: Longitudinal transcriptomic profiling in carrageenan-induced rat hind paw peripheral inflammation and hyperalgesia reveals progressive recruitment of innate immune system components
    Article Snippet: Stained sections were imaged on an Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss, Oberkochen, Germany) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu, Shizuoka, Japan), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies, Waltham MA) and 5 customized filter sets (Semrock, Rochester NY) optimized to detect the following fluorophores: DAPI, Opal520, Opal570, Opal620, Opal690.

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature
    Article Snippet: For multispectral imaging of multiplex immunohistochemistry-stained sections, images were acquired from whole-specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a ×20/0.8 numerical aperture (NA) Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200-W X-Cite 200DC broadband lamp source (Excelitas Technologies) and six customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor-488, Alexa Fluor-546, Alexa Fluor-594, Alexa Fluor-647 and IRDye 800CW.

    Article Title: Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light.
    Article Snippet: Please cite this article as: Matthew R. Sapio, Diana M. King, Dragan Maric, Samay R. Shah, Thomas L. Talbot, Allison P. Manalo, Pranavi Nara, Wenting Ma, Andre Ghetti, Christopher E. Ramsden, Michael J. Iadarola and Andrew J. Mannes, Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light., The Journal of Pain, (2025) doi:https://doi.org/10.1016/j.jpain.2025.105359

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature.
    Article Snippet: Cerebrovascular injury (CVI) is a common pathology caused by infections, injury, stroke, neurodegeneration and autoimmune disease.. Rapid resolution of a CVI requires a coordinated innate immune response.. In the present study, we sought mechanistic insights into how central nervous system-infiltrating monocytes program resident microglia to mediate angiogenesis and cerebrovascular repair after an intracerebral hemorrhage.

    Fluorescence:

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer’s disease
    Article Snippet: Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer's disease
    Article Snippet: Multiplex uorescence immunohistochemistry image acquisition and computational reconstruction Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning uorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized lter sets (Semrock) optimized to detect the following uorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal Page 8/53 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: Neurovascular injury with complement activation and inflammation in COVID-19.
    Article Snippet: Imageswere acquired fromwhole specimen sections using theAxio Imager.Z2 slide scanning fluorescence microscope (Carl Zeiss AG) equipped with a ×20/0.8 Plan-Apochromat (Phase-2) objective (Carl Zeiss AG), an ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies) and eight customized filter sets (Semrock) to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 594, Alexa Fluor 647, IRDye 680LT and IRDye 800CW.

    Article Title: TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression.
    Article Snippet: The nuclear pellet was collected by aspirating ~100 μl from the interface and transferring to a 2.5ml Eppendorf tube.

    Article Title: Longitudinal transcriptomic profiling in carrageenan-induced rat hind paw peripheral inflammation and hyperalgesia reveals progressive recruitment of innate immune system components
    Article Snippet: Stained sections were imaged on an Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss, Oberkochen, Germany) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu, Shizuoka, Japan), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies, Waltham MA) and 5 customized filter sets (Semrock, Rochester NY) optimized to detect the following fluorophores: DAPI, Opal520, Opal570, Opal620, Opal690.

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature
    Article Snippet: For multispectral imaging of multiplex immunohistochemistry-stained sections, images were acquired from whole-specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a ×20/0.8 numerical aperture (NA) Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200-W X-Cite 200DC broadband lamp source (Excelitas Technologies) and six customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor-488, Alexa Fluor-546, Alexa Fluor-594, Alexa Fluor-647 and IRDye 800CW.

    Article Title: Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light.
    Article Snippet: Please cite this article as: Matthew R. Sapio, Diana M. King, Dragan Maric, Samay R. Shah, Thomas L. Talbot, Allison P. Manalo, Pranavi Nara, Wenting Ma, Andre Ghetti, Christopher E. Ramsden, Michael J. Iadarola and Andrew J. Mannes, Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light., The Journal of Pain, (2025) doi:https://doi.org/10.1016/j.jpain.2025.105359

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature.
    Article Snippet: Cerebrovascular injury (CVI) is a common pathology caused by infections, injury, stroke, neurodegeneration and autoimmune disease.. Rapid resolution of a CVI requires a coordinated innate immune response.. In the present study, we sought mechanistic insights into how central nervous system-infiltrating monocytes program resident microglia to mediate angiogenesis and cerebrovascular repair after an intracerebral hemorrhage.

    Microscopy:

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer’s disease
    Article Snippet: Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer's disease
    Article Snippet: Multiplex uorescence immunohistochemistry image acquisition and computational reconstruction Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning uorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized lter sets (Semrock) optimized to detect the following uorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal Page 8/53 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: Neurovascular injury with complement activation and inflammation in COVID-19.
    Article Snippet: Imageswere acquired fromwhole specimen sections using theAxio Imager.Z2 slide scanning fluorescence microscope (Carl Zeiss AG) equipped with a ×20/0.8 Plan-Apochromat (Phase-2) objective (Carl Zeiss AG), an ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies) and eight customized filter sets (Semrock) to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 594, Alexa Fluor 647, IRDye 680LT and IRDye 800CW.

    Article Title: TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression.
    Article Snippet: The nuclear pellet was collected by aspirating ~100 μl from the interface and transferring to a 2.5ml Eppendorf tube.

    Article Title: Longitudinal transcriptomic profiling in carrageenan-induced rat hind paw peripheral inflammation and hyperalgesia reveals progressive recruitment of innate immune system components
    Article Snippet: Stained sections were imaged on an Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss, Oberkochen, Germany) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu, Shizuoka, Japan), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies, Waltham MA) and 5 customized filter sets (Semrock, Rochester NY) optimized to detect the following fluorophores: DAPI, Opal520, Opal570, Opal620, Opal690.

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature
    Article Snippet: For multispectral imaging of multiplex immunohistochemistry-stained sections, images were acquired from whole-specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a ×20/0.8 numerical aperture (NA) Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200-W X-Cite 200DC broadband lamp source (Excelitas Technologies) and six customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor-488, Alexa Fluor-546, Alexa Fluor-594, Alexa Fluor-647 and IRDye 800CW.

    Article Title: Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light.
    Article Snippet: Please cite this article as: Matthew R. Sapio, Diana M. King, Dragan Maric, Samay R. Shah, Thomas L. Talbot, Allison P. Manalo, Pranavi Nara, Wenting Ma, Andre Ghetti, Christopher E. Ramsden, Michael J. Iadarola and Andrew J. Mannes, Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light., The Journal of Pain, (2025) doi:https://doi.org/10.1016/j.jpain.2025.105359

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature.
    Article Snippet: Cerebrovascular injury (CVI) is a common pathology caused by infections, injury, stroke, neurodegeneration and autoimmune disease.. Rapid resolution of a CVI requires a coordinated innate immune response.. In the present study, we sought mechanistic insights into how central nervous system-infiltrating monocytes program resident microglia to mediate angiogenesis and cerebrovascular repair after an intracerebral hemorrhage.

    Imaging:

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer’s disease
    Article Snippet: Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer's disease
    Article Snippet: Multiplex uorescence immunohistochemistry image acquisition and computational reconstruction Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning uorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized lter sets (Semrock) optimized to detect the following uorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal Page 8/53 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: Neurovascular injury with complement activation and inflammation in COVID-19.
    Article Snippet: Imageswere acquired fromwhole specimen sections using theAxio Imager.Z2 slide scanning fluorescence microscope (Carl Zeiss AG) equipped with a ×20/0.8 Plan-Apochromat (Phase-2) objective (Carl Zeiss AG), an ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies) and eight customized filter sets (Semrock) to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 594, Alexa Fluor 647, IRDye 680LT and IRDye 800CW.

    Article Title: TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression.
    Article Snippet: The nuclear pellet was collected by aspirating ~100 μl from the interface and transferring to a 2.5ml Eppendorf tube.

    Article Title: Longitudinal transcriptomic profiling in carrageenan-induced rat hind paw peripheral inflammation and hyperalgesia reveals progressive recruitment of innate immune system components
    Article Snippet: Stained sections were imaged on an Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss, Oberkochen, Germany) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu, Shizuoka, Japan), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies, Waltham MA) and 5 customized filter sets (Semrock, Rochester NY) optimized to detect the following fluorophores: DAPI, Opal520, Opal570, Opal620, Opal690.

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature
    Article Snippet: For multispectral imaging of multiplex immunohistochemistry-stained sections, images were acquired from whole-specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a ×20/0.8 numerical aperture (NA) Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200-W X-Cite 200DC broadband lamp source (Excelitas Technologies) and six customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor-488, Alexa Fluor-546, Alexa Fluor-594, Alexa Fluor-647 and IRDye 800CW.

    Article Title: Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light.
    Article Snippet: Please cite this article as: Matthew R. Sapio, Diana M. King, Dragan Maric, Samay R. Shah, Thomas L. Talbot, Allison P. Manalo, Pranavi Nara, Wenting Ma, Andre Ghetti, Christopher E. Ramsden, Michael J. Iadarola and Andrew J. Mannes, Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light., The Journal of Pain, (2025) doi:https://doi.org/10.1016/j.jpain.2025.105359

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature.
    Article Snippet: Cerebrovascular injury (CVI) is a common pathology caused by infections, injury, stroke, neurodegeneration and autoimmune disease.. Rapid resolution of a CVI requires a coordinated innate immune response.. In the present study, we sought mechanistic insights into how central nervous system-infiltrating monocytes program resident microglia to mediate angiogenesis and cerebrovascular repair after an intracerebral hemorrhage.

    Multiplex Assay:

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer’s disease
    Article Snippet: Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer's disease
    Article Snippet: Multiplex uorescence immunohistochemistry image acquisition and computational reconstruction Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning uorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized lter sets (Semrock) optimized to detect the following uorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal Page 8/53 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: Neurovascular injury with complement activation and inflammation in COVID-19.
    Article Snippet: Imageswere acquired fromwhole specimen sections using theAxio Imager.Z2 slide scanning fluorescence microscope (Carl Zeiss AG) equipped with a ×20/0.8 Plan-Apochromat (Phase-2) objective (Carl Zeiss AG), an ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies) and eight customized filter sets (Semrock) to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 594, Alexa Fluor 647, IRDye 680LT and IRDye 800CW.

    Article Title: TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression.
    Article Snippet: The nuclear pellet was collected by aspirating ~100 μl from the interface and transferring to a 2.5ml Eppendorf tube.

    Article Title: Longitudinal transcriptomic profiling in carrageenan-induced rat hind paw peripheral inflammation and hyperalgesia reveals progressive recruitment of innate immune system components
    Article Snippet: Stained sections were imaged on an Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss, Oberkochen, Germany) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu, Shizuoka, Japan), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies, Waltham MA) and 5 customized filter sets (Semrock, Rochester NY) optimized to detect the following fluorophores: DAPI, Opal520, Opal570, Opal620, Opal690.

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature
    Article Snippet: For multispectral imaging of multiplex immunohistochemistry-stained sections, images were acquired from whole-specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a ×20/0.8 numerical aperture (NA) Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200-W X-Cite 200DC broadband lamp source (Excelitas Technologies) and six customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor-488, Alexa Fluor-546, Alexa Fluor-594, Alexa Fluor-647 and IRDye 800CW.

    Article Title: Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light.
    Article Snippet: Please cite this article as: Matthew R. Sapio, Diana M. King, Dragan Maric, Samay R. Shah, Thomas L. Talbot, Allison P. Manalo, Pranavi Nara, Wenting Ma, Andre Ghetti, Christopher E. Ramsden, Michael J. Iadarola and Andrew J. Mannes, Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light., The Journal of Pain, (2025) doi:https://doi.org/10.1016/j.jpain.2025.105359

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature.
    Article Snippet: Cerebrovascular injury (CVI) is a common pathology caused by infections, injury, stroke, neurodegeneration and autoimmune disease.. Rapid resolution of a CVI requires a coordinated innate immune response.. In the present study, we sought mechanistic insights into how central nervous system-infiltrating monocytes program resident microglia to mediate angiogenesis and cerebrovascular repair after an intracerebral hemorrhage.

    Immunohistochemistry:

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer’s disease
    Article Snippet: Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: ApoER2-Dab1 disruption as the origin of pTau-related neurodegeneration in sporadic Alzheimer's disease
    Article Snippet: Multiplex uorescence immunohistochemistry image acquisition and computational reconstruction Images were acquired from MP-ISH and/or MP-IHC probed specimen sections using the Axio Imager.Z2 slide scanning uorescence microscope (Zeiss) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies), and 8 customized lter sets (Semrock) optimized to detect the following uorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488 (or Opal Page 8/53 520), Alexa Fluor 546 (or Opal 570), Alexa Fluor 594 (or Opal 620), Alexa Fluor 647, IRDye 680LT (or Opal 690), and IRDye 800CW.

    Article Title: Neurovascular injury with complement activation and inflammation in COVID-19.
    Article Snippet: Imageswere acquired fromwhole specimen sections using theAxio Imager.Z2 slide scanning fluorescence microscope (Carl Zeiss AG) equipped with a ×20/0.8 Plan-Apochromat (Phase-2) objective (Carl Zeiss AG), an ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies) and eight customized filter sets (Semrock) to detect the following fluorophores: DAPI, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 546, Alexa Fluor 594, Alexa Fluor 647, IRDye 680LT and IRDye 800CW.

    Article Title: TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression.
    Article Snippet: The nuclear pellet was collected by aspirating ~100 μl from the interface and transferring to a 2.5ml Eppendorf tube.

    Article Title: Longitudinal transcriptomic profiling in carrageenan-induced rat hind paw peripheral inflammation and hyperalgesia reveals progressive recruitment of innate immune system components
    Article Snippet: Stained sections were imaged on an Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss, Oberkochen, Germany) equipped with a 20X/0.8 Plan-Apochromat (Phase-2) non-immersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu, Shizuoka, Japan), a 200W X-Cite 200DC broad band lamp source (Excelitas Technologies, Waltham MA) and 5 customized filter sets (Semrock, Rochester NY) optimized to detect the following fluorophores: DAPI, Opal520, Opal570, Opal620, Opal690.

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature
    Article Snippet: For multispectral imaging of multiplex immunohistochemistry-stained sections, images were acquired from whole-specimen sections using the Axio Imager.Z2 slide scanning fluorescence microscope (Zeiss) equipped with a ×20/0.8 numerical aperture (NA) Plan-Apochromat (Phase-2) nonimmersion objective (Zeiss), a high-resolution ORCA-Flash4.0 sCMOS digital camera (Hamamatsu), a 200-W X-Cite 200DC broadband lamp source (Excelitas Technologies) and six customized filter sets (Semrock) optimized to detect the following fluorophores: DAPI, Alexa Fluor-488, Alexa Fluor-546, Alexa Fluor-594, Alexa Fluor-647 and IRDye 800CW.

    Article Title: Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light.
    Article Snippet: Please cite this article as: Matthew R. Sapio, Diana M. King, Dragan Maric, Samay R. Shah, Thomas L. Talbot, Allison P. Manalo, Pranavi Nara, Wenting Ma, Andre Ghetti, Christopher E. Ramsden, Michael J. Iadarola and Andrew J. Mannes, Efficient removal of naturally-occurring lipofuscin autofluorescence in human nervous tissue using high-intensity white light., The Journal of Pain, (2025) doi:https://doi.org/10.1016/j.jpain.2025.105359

    Article Title: Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature.
    Article Snippet: Cerebrovascular injury (CVI) is a common pathology caused by infections, injury, stroke, neurodegeneration and autoimmune disease.. Rapid resolution of a CVI requires a coordinated innate immune response.. In the present study, we sought mechanistic insights into how central nervous system-infiltrating monocytes program resident microglia to mediate angiogenesis and cerebrovascular repair after an intracerebral hemorrhage.



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